A ratiometric fluorescent probe for hypoxanthine detection in aquatic products based on the enzyme mimics and fluorescence of cobalt-doped carbon nitride

A ratiometric fluorescent probe for hypoxanthine (Hx) detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride (Co doped g-C3N4). In addition to emitting strong fluorescence, peroxidase activity Co g-C3N4 can catalyze reaction O-phenylenediamine H2O2 produce diallyl phthalate which emit yellow at 570 nm. Through decomposition Hx by xanthine oxidase, be indirectly detected generating hydrogen peroxide measurement ratio I (F570/F370). The linear range 1.70 mg kg-1–272 kg-1 (R2=0.997), limit 1.52 (3σ/K, n=9). method applied in bass, grass carp, shrimp, data were verified HPLC. result shows that is sensitive, accurate, reliable, used aquatic products.